dextran hydrodynamic radius

Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Keywords: Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Journal of Applied Polymer Science, 13, 2031-2032. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. 4K, b M.W. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Biomaterials Research Disclaimer. Alternatives to animal testing: a review. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Epub 2017 Jun 1. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Format). Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Harding, S. E., & Simpkin, N. J. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Bookshelf Masuelli, Martin Alberto. Gums and stabilisers for the Food Industry, 5, 447-450. This situation is mainly the result of using dextans, solvent, additives, etc. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Nair SS. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Article Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Dilute Solution. Gekko, K. (1971). For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Schematic illustration of transdermal delivery after the application of the microporation device. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 43-1, 115-147. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Sung-Wook Choi. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Eng. eCollection 2018 Feb. Molecules. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Treatment of acne scars by fractional bipolar radiofrequency energy. Drug deliv. MeSH Carbohydrate Polymers, 74, 372-378. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. (1954). @ Nilimesh See attached. (2003). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. ; Aberle, T; Burchard, W. (2000). Human skins (Full-thickness human skin) were supplied by HansBiomed. and transmitted securely. Front Microbiol. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. Drug Deliv Syst. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Microneedles and their applications. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. This site needs JavaScript to work properly. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. (1991). In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Carbohydrate Polymers, 79, 380-390. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. ; Garca de , J. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Molecular weight, molecular weight distribution and molecular size of a native dextran. For Research Use Only. After 24h, the cumulative permeation of FITC-dextran M.W. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. AATEX. IV. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Nat Biotechnol. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Federal government websites often end in .gov or .mil. Chem. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Biophysical Journal, 27, 177-181. Drug Discov Today Technol. Carbohydrate Polymers 72, 356-360. Pharm Res. Carbohydrate Polymers, 78, 902-907. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Formul. Soc., 16, 3A, 426-433. Radiofrequency ablation of malignant liver tumors. European Biophysical Journal, 36, 85-94. Hindered diffusion of dextran and ficoll in microporous membranes. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. International Journal of Pharmaceutics, 277, 99-104. Chem. J. Braz. Epub 2022 Feb 2. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Walkenhorst, S.; Olivier, R. (2003). Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Pharmacol Pharm. J. Phys. Polyelectrolytes as secondary calibration standards for aqueous SEC. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). On the molecular weight distribution of dextran T-500. CAS Kasaai, Mohammad R. (2012). The human skins were from the back or thigh of a 24-year-old woman. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Carbohydrate Polymers, 38, 267-271. sharing sensitive information, make sure youre on a federal Rheology of dextran solutions. Dilute solution properties and degree of chain branching for dextran. ; van Nostrum, C.F. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Journal of Physical Chemistry, 58(11), 953-957. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). The Viscosity of Dilute Solutions of Long-Chain Molecules. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. official website and that any information you provide is encrypted TEWL measurements were taken 30min after microporation of the human and pig skin. Carbohydrate Polymers, 36, 319-326. TEWL measurements were performed before and 30min after microporation. government site. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Chem. Global hydrodynamic analysis of the molecular flexibility of galactomannans. HHS Vulnerability Disclosure, Help Advances in Cosmetic Surgery. Nordmeier, Eckliard (1993). 2009;8(2):1739. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. dextran hydrodynamic radius. International Journal of Biological Macromolecules, 23, 135-141. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Hydrodynamic radii ( RH) were determined by dynamic light scattering. of different origins. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the (2008). Smith, David R.; Raymonda, John W. (1972). 2010;4(1):117. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Journal of Chemical Education, 49(8), 577-579. The https:// ensures that you are connecting to the Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. By using this website, you agree to our International Journal of Biological Macromolecules, 42, 133-137. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. All authors have consented to the submission of this manuscript for publication. ; Patel, T.R. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Int. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. 2003;10:33847. Proc. (1954). Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Cookies policy. Molecular weight determination of polysaccharides. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Critical parameters for gel permeation Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Google Scholar. Curley SA. Learn More. Thermo Fisher Scientific. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. H&E stained images were analyzed using Image J software. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Crini, G. (2005). Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. alachua county spring break 2022,